GUT & ESTROBOLOME· 🌀 The Second Axis

Gut bacteria control how much estrogen stays in circulation. The estrobolome is real.

When gut dysbiosis increases beta-glucuronidase activity, conjugated estrogens are deconjugated and reabsorbed instead of excreted. The result is an estrogen load the liver didn't account for — driving cycle disruption, heavy periods, and endometriosis flares that no endocrine protocol alone can fix.

The Second Axis targets the gut-hormone connection from both directions. A high-potency Lactobacillus probiotic blend reduces dysbiotic beta-glucuronidase activity. Calcium D-glucarate and DIM support hepatic estrogen conjugation and clearance. Psyllium husk binds conjugated estrogens in the gut to prevent reabsorption. Magnesium glycinate and omega-3 address the inflammatory gut-lining disruption that makes the estrobolome cycle worse.

Build my profile — freeSee your pack ↓

Free to start · from $49/mo with delivery · NSF tested · 100% vegan

“Plottel CS & Blaser MJ's landmark 2011 paper in Science Translational Medicine defined the estrobolome concept: the aggregate of gut bacterial genes encoding beta-glucuronidase is capable of deconjugating estrogens, allowing their reabsorption into the entero-hepatic circulation. In women with estrogen-dominant conditions — endometriosis, PCOS, fibroids, severe PMS — dysbiosis measurably increases circulating estrogen. The estrobolome is bidirectional: estrogen also modulates gut microbiome diversity, with lower estrogen (post-menopause) associated with reduced Lactobacillus populations. Baker JM et al. 2017 further quantified the relationship between microbiome composition and urinary estrogen metabolite ratios.”

Plottel CS & Blaser MJ, Science Translational Medicine, 2011

YOUR PROFILE

🌀

The Second Axis

Gut & Estrobolome

WHAT'S IN YOUR PACK

Lactobacillus acidophilus + L. reuteri probiotic blend50B CFU
Lactobacillus species — particularly L. acidophilus and L. reuteri — produce lower beta-glucuronidase activity than dysbiotic populations (Bacteroides, Clostridia) that dominate in poor gut health. High-potency multi-strain blends at 50B CFU have stronger evidence for reducing IBS symptoms, restoring microbial diversity, and reducing estrobolome dysbiosis markers than lower-dose single-strain products. Take with food to improve survival through gastric acid.
Calcium D-glucarate500mg
Calcium D-glucarate is a natural compound that inhibits beta-glucuronidase activity in the gut — directly slowing the deconjugation and reabsorption of estrogens the liver worked to clear. Walaszek Z et al. 1997: calcium D-glucarate reduced serum estrogen levels in animal models; human data supports its use in estrogen clearance support. This is the most direct intervention for the estrobolome mechanism.
DIM (diindolylmethane)100mg
DIM is the bioactive compound formed from I3C (indole-3-carbinol) in cruciferous vegetables — the reason broccoli has cancer-prevention associations. At 100mg, DIM shifts hepatic estrogen metabolism toward the 2-hydroxyestrone pathway (less proliferative) and away from the 16-alpha-hydroxyestrone pathway (more proliferative). This is estrogen quality optimization rather than estrogen reduction. Avoid during pregnancy or when trying to conceive.
Psyllium husk5g
Psyllium is a soluble fiber that binds conjugated estrogens in the gut lumen, physically preventing their reabsorption before beta-glucuronidase can deconjugate them. It also feeds Lactobacillus species preferentially, supporting the probiotic population this stack is trying to build. Psyllium at 5g daily also normalizes stool transit time — slow transit gives beta-glucuronidase more time to work.
Magnesium glycinate300mg
Magnesium deficiency is associated with increased intestinal permeability ("leaky gut") and elevated inflammatory cytokines in the gut lining. Adequate magnesium supports the tight junction proteins (ZO-1, occludin) that maintain gut barrier integrity — the same barrier that dysbiosis degrades. Glycinate form avoids the GI discomfort that limits higher doses of oxide or citrate.
Omega-3 EPA/DHA1g
EPA reduces intestinal inflammation via the prostaglandin E3 pathway — counteracting the pro-inflammatory arachidonic acid cascade that dysbiosis amplifies. At 1g, this is a gut-targeted maintenance dose (the cardiovascular dose is 2–3g). Omega-3 supplementation has also been associated with increased microbial diversity in human trials, particularly Bifidobacterium and Lactobacillus populations.

Take probiotics with food to improve survival through gastric acid. DIM and calcium D-glucarate affect estrogen metabolism — avoid if pregnant or trying to conceive without guidance from your provider. Start probiotics at a lower dose if you are not already taking them regularly, then build up to 50B CFU over two to three weeks to minimize adjustment GI symptoms (bloating, gas).

“I had estrogen dominance symptoms for years — heavy periods, bloating, PMS that lasted two weeks. Three different gynecologists told me my estrogen levels were normal. What none of them mentioned was that my gut was recirculating it. The Second Axis — especially the calcium D-glucarate and the probiotic — changed what my late cycle looked like within about two months.”

Rachel M., 33
Estrogen dominance pattern · history of IBS · Austin

COMMON QUESTIONS

Build your
The Second Axis profile.

Two minutes. Completely free. Your profile and pack preview are yours before you ever enter a card.

Begin free →

FOUNDING MEMBERS

500 spots. Price locked forever. Essential $39/mo, Full Protocol $69/mo — permanent founding rate.

RELATED PROFILES

PCOS →Endometriosis →Thyroid →All 33 profiles →